Generation of bicistronic reporter knockin mice for visualizing germ layers
نویسندگان
چکیده
منابع مشابه
Systematic analysis of bicistronic reporter assay data.
Bicistronic reporter assay systems have become a mainstay of molecular biology. While the assays themselves encompass a broad range of diverse and unrelated experimental protocols, the numerical data garnered from these experiments often have similar statistical properties. In general, a primary dataset measures the paired expression of two internally controlled reporter genes. The expression r...
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Deciphering the etiology of complex pathologies at molecular level requires longitudinal studies encompassing multiple biochemical pathways (apoptosis, proliferation, inflammation, oxidative stress). In vivo imaging of current reporter animals enabled the spatio-temporal analysis of specific molecular events, however, the lack of a multiplicity of loci for the generalized and regulated expressi...
متن کاملGerm Layers
A germ layer is a group of cells in an embryo that interact with each other as the embryo develops and contribute to the formation of all organs and tissues. All animals, except perhaps sponges, form two or three germ layers [6]. The germ layers [6] develop early in embryonic life, through the process of gastrulation [7]. During gastrulation [7], a hollow cluster of cells called a blastula [8] ...
متن کاملGerm Layers
A germ layer is a group of cells in an embryo that interact with each other as the embryo develops and contribute to the formation of all organs and tissues. All animals, except perhaps sponges, form two or three germ layers [6]. The germ layers [6] develop early in embryonic life, through the process of gastrulation [7]. During gastrulation [7], a hollow cluster of cells called a blastula [8] ...
متن کاملFucci2a: A bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice
Markers of cell cycle stage allow estimation of cell cycle dynamics in cell culture and during embryonic development. The Fucci system incorporates genetically encoded probes that highlight G1 and S/G2/M phases of the cell cycle allowing live imaging. However the available mouse models that incorporate Fucci are beset by problems with transgene inactivation, varying expression level, lack of co...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 2019
ISSN: 1341-1357,1881-7122
DOI: 10.1538/expanim.19-0031